The vocal crowd of one hundred and twenty six were treated to an enthralling second-half encounter.
Eastbourne shot out of the blocks with Archie Collett racing away from the AFC defence to get on the end of Bateman’s long ball over the top but he fired straight at Beasley when well placed.
Minutes later Collett spurned another opportunity for Boro as his shot from the edge of the area flew over the crossbar.
Fifteen minutes into the second period and the game started to have a more open feel to it. Langan again tested Lankston’s handling with a strike at goal which the Eastbourne keeper gathered at the second attempt.
Straight down the other end Hobden, who was causing all sorts of problems for the Varndeanians back line, turned inside his marker brilliantly before firing straight at Beasley.
Then, on the hour mark, Eastbourne were awarded a penalty. Hobden chased a lofted ball and as he looked to latch on to it inside the area he was wiped out by Beasley leaving the referee no option but to point to the spot. Cook stepped up and dispatched the penalty with aplomb.
Boro had a fantastic opportunity to double their lead a couple of minutes later. An in-swinging corner was met on the volley by skipper Daniel Blackmore four yards out, but off balance he could only direct his effort up and over Lankston’s crossbar.
With just six minutes remaining the Varndeanians equalised. A Boro goal-kick was headed back the way it came by Kaine Starks and Rex Lane was in, the substitute kept his cool to slot the ball low past the advancing Lankston and into the net.
The game looked to be heading for extra-time but AFC had other ideas. Langan forced Lankston into fine one handed diving save before Marley Ambler flipped the game on its head leaving the Eastbourne players and supporters stunned.
Ambler strode forward with the ball and rolled in Lane, his low shot was save by Lankston, but only into the path of Ambler, who had continued his run into the box and he tucked the ball past Blackmore on the line to spark jubilant celebrations in the main stand.
Following the match victorious AFC Varndeanians manager, Darren Barker, said: “It was a very, very good game. We were up against it and probably weren’t the best team in the first-half, but we done brilliantly to pull through in second.
“After going 1-0 down we showed so much character to bring it back and turn the game around, it was absolutely fantastic, I’m delighted!”